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Tubulin polymerization modulates interleukin-2 receptor signal transduction in human T cells.
- Source :
-
Journal of receptor and signal transduction research [J Recept Signal Transduct Res] 2006; Vol. 26 (1-2), pp. 87-106. - Publication Year :
- 2006
-
Abstract
- Few data exist on the modulation of cytokine receptor signaling by the actin or tubulin cytoskeleton. Therefore, we studied interleukin-2 receptor (IL-2R) signaling in phytohemagglutinine (PHA)-pretreated human T cells in the context of alterations in the cytoskeletal system induced by cytochalasin D (CyD), jasplaklinolide (Jas), taxol (Tax), or colchicine (Col). We found that changes in cytoskeletal tubulin polymerization altered the strength of several IL-2-triggered signals. Moreover, Tax-induced tubulin hyperpolymerization augmented the surface expression of the IL-2R ss -chain and enhanced the association of the IL-2R beta -chain with cytoskeletal tubulin. The IL-2R beta-chain, in turn, was constitutively associated with tubulin and, more weakly, actin. To exclude the possibility that these associations are artifacts caused by PHA, we confirmed them in T cells from TCR-transgenic DO 11.10 mice stimulated with their nominal antigen. We conclude that altered polymerization of cytoskeletal components, especially tubulin, is accompanied by modulation of IL-2 signaling at the receptor level.
- Subjects :
- Actins metabolism
Animals
Antineoplastic Agents pharmacology
Antineoplastic Agents, Phytogenic pharmacology
Colchicine pharmacology
Cytochalasin D pharmacology
Cytoskeleton metabolism
Depsipeptides pharmacology
Gout Suppressants pharmacology
Humans
Interleukin-2 pharmacology
Mice
Mice, Transgenic
Mitogens pharmacology
Nucleic Acid Synthesis Inhibitors pharmacology
Paclitaxel pharmacology
Phytohemagglutinins pharmacology
Receptors, Interleukin-2 metabolism
Signal Transduction
T-Lymphocytes metabolism
Tubulin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1079-9893
- Volume :
- 26
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of receptor and signal transduction research
- Publication Type :
- Academic Journal
- Accession number :
- 16595340
- Full Text :
- https://doi.org/10.1080/10799890600567372