Back to Search
Start Over
Cyclin D1 antisense oligodexoyneucleotides inhibits growth and enhances chemosensitivity in gastric carcinoma cells.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2006 Mar 21; Vol. 12 (11), pp. 1766-9. - Publication Year :
- 2006
-
Abstract
- Aim: To examine the effects of cyclin D1 antisense oligodexoyneucleotides (ASODN) on growth and chemosensitivity of gastric carcinoma cell lines SGC7901 and its mechanism.<br />Methods: Phosphorothioate modified cyclin D1 ASODN was encapsulated by LipofectAMINE2000 (LF2000) and transfected into cells, the dose-effect curves and growth curves were observed. 5-FU, MTX, CDDP of different concentrations were given after transfecting cells with cyclin D1 ASODN for 24 h the dose-effect responses were observed and IC50s were calculated. The mRNA expression of cyclin D1, thymidylate synthase (TS), thymidine phosphorylase (TP) and dihydrofolate reductase (DHFR) was detected by reverse transcription-PCR (RT-PCR) at 24 h and 48 h after transfection.<br />Results: Dose-dependent inhibitory effect was caused by cyclin D1 ASODN in SGC7901 cells. Transfecting gastric carcinoma cells with 0.2 micromol/L cyclin D1 ASODN for 24 h could inhibit growth significantly and reduce expression of cyclin D1 mRNA. Cyclin D1 ASODN could increase the chemosensitivity to 5-FU, MTX, CDDP in cells. The IC50s of different chemotherapeutic agents in ASODN plus chemotherapy groups were significantly lower than those in controls. Transfection with cyclin D1 ASODN leaded to an increase in TS and DHFR mRNA and a decrease in TP mRNA as determined by RT-PCR at 24 h, the alterations were more significant at 48 h.<br />Conclusions: Cyclin D1 ASODN can decrease mRNA expression of cyclin D1, inhibit growth and enhance the chemosensitivity by changing the expression of enzymes related to metabolism of chemotherapeutic agents in SGC7901 gastric carcinoma cells.
- Subjects :
- Adenocarcinoma drug therapy
Adenocarcinoma metabolism
Cell Line, Tumor
Cell Proliferation drug effects
Chemoreceptor Cells drug effects
Cisplatin pharmacology
Cyclin D1 physiology
Dose-Response Relationship, Drug
Fluorouracil pharmacology
Gene Expression Regulation, Enzymologic drug effects
Gene Expression Regulation, Neoplastic drug effects
Humans
Methotrexate pharmacology
RNA, Messenger analysis
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms drug therapy
Stomach Neoplasms metabolism
Tetrahydrofolate Dehydrogenase genetics
Tetrahydrofolate Dehydrogenase physiology
Thymidine Phosphorylase genetics
Thymidine Phosphorylase physiology
Thymidylate Synthase genetics
Thymidylate Synthase physiology
Transfection
Adenocarcinoma pathology
Antineoplastic Agents pharmacology
Cyclin D1 genetics
Oligodeoxyribonucleotides, Antisense pharmacology
Stomach Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 12
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 16586549
- Full Text :
- https://doi.org/10.3748/wjg.v12.i11.1766