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Engineered heart tissue grafts improve systolic and diastolic function in infarcted rat hearts.
- Source :
-
Nature medicine [Nat Med] 2006 Apr; Vol. 12 (4), pp. 452-8. Date of Electronic Publication: 2006 Apr 02. - Publication Year :
- 2006
-
Abstract
- The concept of regenerating diseased myocardium by implantation of tissue-engineered heart muscle is intriguing, but convincing evidence is lacking that heart tissues can be generated at a size and with contractile properties that would lend considerable support to failing hearts. Here we created large (thickness/diameter, 1-4 mm/15 mm), force-generating engineered heart tissue from neonatal rat heart cells. Engineered heart tissue formed thick cardiac muscle layers when implanted on myocardial infarcts in immune-suppressed rats. When evaluated 28 d later, engineered heart tissue showed undelayed electrical coupling to the native myocardium without evidence of arrhythmia induction. Moreover, engineered heart tissue prevented further dilation, induced systolic wall thickening of infarcted myocardial segments and improved fractional area shortening of infarcted hearts compared to controls (sham operation and noncontractile constructs). Thus, our study provides evidence that large contractile cardiac tissue grafts can be constructed in vitro, can survive after implantation and can support contractile function of infarcted hearts.
- Subjects :
- Animals
Animals, Newborn
Calcium pharmacology
Dose-Response Relationship, Drug
Echocardiography
Electric Stimulation
Fluorescent Dyes
Heart drug effects
Indoles
Insulin pharmacology
Isometric Contraction drug effects
Magnetic Resonance Imaging
Male
Microscopy, Confocal
Myocardial Contraction physiology
Myocardial Infarction etiology
Myocardium cytology
Myocytes, Cardiac physiology
Oxygen pharmacology
Rats
Rats, Wistar
Time Factors
Ventricular Function, Left
Heart Transplantation methods
Myocardial Infarction pathology
Systole
Tissue Engineering methods
Transplants
Subjects
Details
- Language :
- English
- ISSN :
- 1078-8956
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nature medicine
- Publication Type :
- Academic Journal
- Accession number :
- 16582915
- Full Text :
- https://doi.org/10.1038/nm1394