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High-resolution physical mapping and construction of a porcine contig spanning the intramuscular fat content QTL.
- Source :
-
Animal genetics [Anim Genet] 2006 Apr; Vol. 37 (2), pp. 113-20. - Publication Year :
- 2006
-
Abstract
- We previously mapped a locus for porcine intramuscular fat content (IMF) by linkage analysis to a 17.1-cM chromosome interval on Sus scrofa chromosome 7 (SSC7) flanked by microsatellite markers SW1083 and SW581. In this study, we identified 34 microsatellite markers and 14 STSs from the 17.1-cM IMF quantitative trait loci (QTL) region corresponding to HSA14q and aligned those loci using the INRA-University of Minnesota porcine radiation hybrid (IMpRH) panel. We then constructed a 5.2-Mb porcine bacterial artificial chromosome (BAC) contig of this region that was aligned using the RH panel. Finally, the IMF QTL was fine-mapped to 12.6 cM between SJ169 and MM70 at the 0.1% chromosome-wise significance level by genotyping the previously studied F2 resource family with 17 additional microsatellites. We also demonstrated that the SJ169-MM70 interval spans approximately 3.0 Mb and contains at least 12 genes: GALC, GPR65, KCNK10, SPATA7, PTPN21, FLJ11806, EML5, TTC8, CHES1, CAP2P1, CHORDC2P and C14orf143.
Details
- Language :
- English
- ISSN :
- 0268-9146
- Volume :
- 37
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Animal genetics
- Publication Type :
- Academic Journal
- Accession number :
- 16573525
- Full Text :
- https://doi.org/10.1111/j.1365-2052.2005.01397.x