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Effect of glutamine on glutathione kinetics in vivo in dogs.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2007 Jan; Vol. 18 (1), pp. 10-6. Date of Electronic Publication: 2006 Mar 24. - Publication Year :
- 2007
-
Abstract
- To determine whether glutamine affects glutathione (GSH, gamma-glutamyl-cysteinyl-glycine) metabolism, seven healthy beagle dogs received 6-h infusions of [(15)N]glutamate and [(13)C]leucine after a 3-day fast. Isotope infusions were performed during oral feeding with an elemental regimen, supplemented with either l-glutamine or an isonitrogenous amino acid mixture, on two separate days and in randomized order. Timed blood samples were obtained, and a surgical duodenal biopsy was performed after 6 h of isotope infusion. GSH fractional synthesis rate (FSR) was assessed from [(15)N]glutamate incorporation into blood and gut GSH, and duodenal protein synthesis from [(13)C]leucine incorporation into gut protein. Glutamine supplementation failed to alter erythrocyte GSH concentration (2189+/-86 vs. 1994+/-102 micromol L(-1) for glutamine vs. control; ns) or FSR (64+/-17% vs. 74+/-20% day(-1); ns). In the duodenum, glutamine supplementation was associated with a 92% rise in reduced/oxidized GSH ratio (P=.024) and with a 44% decline in GSH FSR (96+/-15% day(-1) vs. 170+/-18% day(-1); P=.005), whereas total GSH concentration remained unchanged (808+/-154 vs. 740+/-127 micromol kg(-1); P=.779). We conclude that, in dogs receiving enteral nutrition after a 3-day fast: (1) glutamine availability does not affect blood GSH, and, (2) in contrast, in the duodenum, the preserved GSH pool, along with a decreased synthesis rate, suggests that glutamine may maintain GSH pool and intestinal redox status by acutely decreasing GSH utilization.
- Subjects :
- Animals
Dogs
Duodenum metabolism
Enteral Nutrition
Erythrocytes chemistry
Female
Glutamic Acid blood
Glutamine blood
Glutathione biosynthesis
Glutathione blood
Intestinal Mucosa metabolism
Kinetics
Male
Models, Animal
Oxidation-Reduction
Protein Biosynthesis
Glutamine pharmacology
Glutathione metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0955-2863
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16563721
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2006.02.002