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Effect of specific enzyme inhibitors on replication, total genome DNA repair and on gene-specific DNA repair after UV irradiation in CHO cells.
- Source :
-
Mutation research [Mutat Res] 1991 Sep; Vol. 255 (2), pp. 155-62. - Publication Year :
- 1991
-
Abstract
- We have studied the effect of some specific enzyme inhibitors on DNA repair and replication after UV damage in Chinese hamster ovary cells. The DNA repair was studied at the level of the average, overall genome and also in the active dihydrofolate reductase gene. Replication was measured in the overall genome. We tested inhibitors of DNA polymerase alpha and delta (aphidicolin), of poly(ADPr) polymerase (3-aminobenzamide), of ribonucleotide reductase (hydroxyurea), of topoisomerase I (camptothecin), and of topoisomerase II (merbarone, VP-16). In addition, we tested the effect of the potential topoisomerase I activator, beta-lapachone. All of these compounds inhibited genome replication and all topoisomerase inhibitors affected the overall genome repair; beta-lapachone stimulated it. None of these compounds had any effect on the gene-specific repair.
- Subjects :
- Animals
Blotting, Southern
CHO Cells
Cricetinae
DNA Replication radiation effects
Nucleic Acid Synthesis Inhibitors
Tetrahydrofolate Dehydrogenase genetics
Topoisomerase I Inhibitors
Topoisomerase II Inhibitors
DNA Repair drug effects
DNA Repair radiation effects
DNA Replication drug effects
Enzyme Inhibitors pharmacology
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 255
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 1656249
- Full Text :
- https://doi.org/10.1016/0921-8777(91)90049-u