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Picornavirus infections in children diagnosed by RT-PCR during longitudinal surveillance with weekly sampling: Association with symptomatic illness and effect of season.
- Source :
-
Journal of medical virology [J Med Virol] 2006 May; Vol. 78 (5), pp. 644-50. - Publication Year :
- 2006
-
Abstract
- RT-PCR is more sensitive for rhinovirus detection than cell culture, but healthy controls are frequently rhinovirus (or picornavirus) positive in cross-sectional studies. Fifteen healthy children were followed over at least three seasons of the year with weekly sampling of nasal/nasopharyngeal secretion for RT-PCR testing for picornavirus and daily recording of respiratory symptoms. One sample positive for picornavirus was diagnosed as an infection; consecutive positive weekly samples constituted a single infection. Picornavirus illness was diagnosed if RNA was detected 7 days prior through 21 days after onset. One hundred fifty-five (21%) of 740 weekly samples were picornavirus positive and associated with illness; 37(5%) positives were not associated with illness (P = 0.001). The 192 positive samples occurred in 121 infections, 74 with a single positive and 47 with "runs" of positives in two or more consecutive samples. Forty five (96%) of the 47 runs comprised 2 or 3 consecutive positives. Ninety six (52%) of 185 reported illnesses during 235 child-months were picornavirus positive (0.4/child-month); 25 infections were asymptomatic (0.11/child-month). The infection rate was highest in fall (0.66/child-month); the winter rate (0.44/child-month) was similar to that in spring (0.5) and summer (0.43). Picornavirus infections in healthy children were common (0.51/child-month), episodic, and usually associated with brief illness; one fifth of infections were asymptomatic. The infection rate was highest in fall; infections in winter occurred at the same rate as in spring and summer.<br /> (Copyright 2006 Wiley-Liss, Inc.)
- Subjects :
- Carrier State virology
Child
Child, Preschool
Humans
Infant
Longitudinal Studies
Nasal Mucosa virology
Pharynx virology
Picornaviridae genetics
Picornaviridae Infections diagnosis
Polymerase Chain Reaction
RNA, Viral genetics
Respiratory Mucosa virology
Respiratory Tract Infections diagnosis
Respiratory Tract Infections virology
Seasons
Time Factors
United States epidemiology
Carrier State epidemiology
Picornaviridae isolation & purification
Picornaviridae Infections epidemiology
Respiratory Tract Infections epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 0146-6615
- Volume :
- 78
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 16555289
- Full Text :
- https://doi.org/10.1002/jmv.20588