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AROS-29 is involved in adaptive response to oxidative stress.
- Source :
-
Free radical research [Free Radic Res] 2006 May; Vol. 40 (5), pp. 467-76. - Publication Year :
- 2006
-
Abstract
- Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H(2)O(2)) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.
- Subjects :
- Adaptation, Physiological drug effects
Apoptosis physiology
Blotting, Western
Cell Line, Tumor
Comet Assay
Flow Cytometry
Gene Expression
Gene Expression Profiling
Glutathione drug effects
Glutathione metabolism
Humans
Maleates pharmacology
Membrane Proteins genetics
Oxidative Stress drug effects
RNA, Messenger analysis
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Adaptation, Physiological physiology
Membrane Proteins metabolism
Oxidative Stress physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1071-5762
- Volume :
- 40
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 16551573
- Full Text :
- https://doi.org/10.1080/10715760600570547