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Induction of systemic and mucosal immune response and decrease in Streptococcus pneumoniae colonization by nasal inoculation of mice with recombinant lactic acid bacteria expressing pneumococcal surface antigen A.
- Source :
-
Microbes and infection [Microbes Infect] 2006 Apr; Vol. 8 (4), pp. 1016-24. Date of Electronic Publication: 2006 Jan 18. - Publication Year :
- 2006
-
Abstract
- Mucosal epithelia constitute the first barriers to be overcome by pathogens during infection. The induction of protective IgA in this location is important for the prevention of infection and can be achieved through different mucosal immunization strategies. Lactic acid bacteria have been tested in the last few years as live vectors for the delivery of antigens at mucosal sites, with promising results. In this work, Streptococcus pneumoniae PsaA antigen was expressed in different species of lactic acid bacteria, such as Lactococcus lactis, Lactobacillus casei, Lactobacillus plantarum, and Lactobacillus helveticus. After nasal inoculation of C57Bl/6 mice, their ability to induce both systemic (IgG in serum) and mucosal (IgA in saliva, nasal and bronchial washes) anti-PsaA antibodies was determined. Immunization with L. lactis MG1363 induced very low levels of IgA and IgG, possibly by the low amount of PsaA expressed in this strain and its short persistence in the nasal mucosa. All three lactobacilli persisted in the nasal mucosa for 3 days and produced a similar amount of PsaA protein (150-250 ng per 10(9) CFU). However, L. plantarum NCDO1193 and L. helveticus ATCC15009 elicited the highest antibody response (IgA and IgG). Vaccination with recombinant lactobacilli but not with recombinant L. lactis led to a decrease in S. pneumoniae recovery from nasal mucosa upon a colonization challenge. Our results confirm that certain Lactobacillus strains have intrinsic properties that make them suitable candidates for mucosal vaccination experiments.
- Subjects :
- Adhesins, Bacterial biosynthesis
Adhesins, Bacterial genetics
Administration, Intranasal
Amino Acid Sequence
Animals
Antibodies, Bacterial blood
Bacterial Adhesion genetics
Bacterial Proteins genetics
Female
Immunoglobulin A analysis
Immunoglobulin G blood
Lactobacillus genetics
Lactobacillus metabolism
Lipoproteins biosynthesis
Lipoproteins genetics
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Protein Sorting Signals genetics
Saliva immunology
Species Specificity
Adhesins, Bacterial immunology
Antibodies, Bacterial analysis
Bacterial Adhesion immunology
Lipoproteins immunology
Pneumococcal Infections immunology
Pneumococcal Infections prevention & control
Pneumococcal Vaccines administration & dosage
Respiratory Mucosa immunology
Streptococcus pneumoniae immunology
Vaccination
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1286-4579
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microbes and infection
- Publication Type :
- Academic Journal
- Accession number :
- 16549380
- Full Text :
- https://doi.org/10.1016/j.micinf.2005.10.020