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Characterization of semenogelin proteins in the human retina.
- Source :
-
Experimental eye research [Exp Eye Res] 2006 Jul; Vol. 83 (1), pp. 120-7. Date of Electronic Publication: 2006 Mar 20. - Publication Year :
- 2006
-
Abstract
- Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell lysates. Immunoreactivity was detected in the ganglion cell and photoreceptor layer of the retina. Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress.
- Subjects :
- Aged
Aged, 80 and over
Choroid chemistry
Humans
Immunohistochemistry methods
Middle Aged
Photoreceptor Cells, Vertebrate chemistry
Pigment Epithelium of Eye chemistry
Proteomics methods
Retina cytology
Retinal Ganglion Cells chemistry
Vitreous Body chemistry
Eye Proteins analysis
Retina chemistry
Seminal Vesicle Secretory Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0014-4835
- Volume :
- 83
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental eye research
- Publication Type :
- Academic Journal
- Accession number :
- 16545373
- Full Text :
- https://doi.org/10.1016/j.exer.2005.11.011