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DNA vaccines expressing pneumococcal surface protein A (PspA) elicit protection levels comparable to recombinant protein.
- Source :
-
Journal of medical microbiology [J Med Microbiol] 2006 Apr; Vol. 55 (Pt 4), pp. 375-378. - Publication Year :
- 2006
-
Abstract
- Pneumococcal surface protein A (PspA) is a promising candidate for the development of cost-effective vaccines against Streptococcus pneumoniae. In the present study, BALB/c mice were immunized with DNA vaccine vectors expressing the N-terminal region of PspA. Animals immunized with a vector expressing secreted PspA developed higher levels of antibody than mice immunized with the vector expressing the antigen in the cytosol. However, both immunogens elicited similar levels of protection against intraperitoneal challenge. Furthermore, immunization with exactly the same fragment in the form of a recombinant protein, with aluminium hydroxide as an adjuvant, elicited even higher antibody levels, but this increased humoral response did not correlate with enhanced protection. These results show that DNA vaccines expressing PspA are able to elicit protection levels comparable to recombinant protein, even though total anti-PspA IgG response is considerably lower.
- Subjects :
- Animals
Antibodies, Bacterial blood
Female
Immunoglobulin G blood
Mice
Mice, Inbred BALB C
Pneumococcal Infections prevention & control
Pneumococcal Vaccines genetics
Bacterial Proteins genetics
Bacterial Proteins immunology
Pneumococcal Vaccines immunology
Recombinant Proteins administration & dosage
Recombinant Proteins immunology
Vaccines, DNA genetics
Vaccines, DNA immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2615
- Volume :
- 55
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- Journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16533983
- Full Text :
- https://doi.org/10.1099/jmm.0.46217-0