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Microarray analysis of PDGFR alpha+ populations in ES cell differentiation culture identifies genes involved in differentiation of mesoderm and mesenchyme including ARID3b that is essential for development of embryonic mesenchymal cells.
- Source :
-
Developmental biology [Dev Biol] 2006 May 01; Vol. 293 (1), pp. 25-37. Date of Electronic Publication: 2006 Mar 13. - Publication Year :
- 2006
-
Abstract
- An inherent difficulty in using DNA microarray technology on the early mouse embryo is its relatively small size. In this study, we investigated whether use of ES cell differentiation culture, which has no theoretical limit in the number of cells that can be generated, can improve this situation. Seven distinct ES-cell-derived populations were analyzed by DNA microarray and examined for genes whose distribution patterns are similar to those of PDGFRalpha, a gene implicated in differentiation of mesoderm/mesenchymal lineages. Using software developed in our laboratory, we formed a group of 30 genes which showed the highest similarity to PDGFRalpha, 18 of these genes were shown to be involved in development of either mesodermal, mesenchymal or neural crest cells. This list also contains several genes whose role in embryogenesis has not yet been fully identified. One such molecule is mARID3b. The mARID3b expression is found in the paraxial mesoderm and cranial mesenchyme. mARID3b-null mouse showed early embryonic lethality, and most phenotypes of this mutant appear to develop from a failure to generate a sufficient number of cranial mesenchymal cells. These results demonstrate the potential use of ES cell differentiation culture in identifying novel genes playing an indispensable role in embryogenesis.
- Subjects :
- Animals
Cells, Cultured
DNA-Binding Proteins biosynthesis
Gene Expression Profiling
Growth Substances physiology
Head embryology
Mesoderm metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Oligonucleotide Array Sequence Analysis
Receptor, Platelet-Derived Growth Factor alpha biosynthesis
Stem Cells metabolism
Cell Differentiation genetics
DNA-Binding Proteins genetics
Growth Substances genetics
Mesoderm cytology
Receptor, Platelet-Derived Growth Factor alpha genetics
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0012-1606
- Volume :
- 293
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 16530748
- Full Text :
- https://doi.org/10.1016/j.ydbio.2005.12.016