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T-lymphocyte reconstitution following rigorously T-cell-depleted versus unmodified autologous stem cell transplants.
- Source :
-
Bone marrow transplantation [Bone Marrow Transplant] 2006 Apr; Vol. 37 (8), pp. 763-72. - Publication Year :
- 2006
-
Abstract
- We compared the kinetics of T-cell recovery after extensive ex vivo and in vivo T-cell depleted autologous stem cell transplantation (SCT) for multiple sclerosis (MS; n=8) with unmodified SCT for hematological malignancies (HM; n=39). Both patient group showed a very protracted recovery of 'naive' CD4(+), 45R0(-) ( approximately CD45RA(+)) T-cells. Within the 'primed' CD4(+), 45R0(+) T-cells, the 'central memory' cells expressing the CD62L and CD27 markers were the slowest to recover. The repopulating T-cells were highly activated, as shown by increased expression of HLA-DR and the apoptosis marker CD95. The capability of CD4(+) and CD8(+) T-cells to produce IFN-gamma, IL-2 and TNF-alpha had reached normal ranges from 2 months post SCT onwards. Unexpectedly, the kinetics of T-cell recovery between 3 and 12 months post transplant was similar in T-depleted and unmodified SCT. Before SCT, the HM patients showed lymphopenia of all T-cell subsets, upregulated HLA-DR and CD95 expression and increased cytokine responses. We suggest that the similar kinetics of T-cell recovery in the two patient groups may be explained by the susceptibility to apoptosis of the activated CD4(+) T-cells in the autografts of the HM patients. This susceptibility to apoptosis would interfere with a swift and sustained CD4(+) T-cell regeneration post SCT.
- Subjects :
- Adult
Apoptosis
CD3 Complex biosynthesis
CD4 Antigens biosynthesis
CD4-Positive T-Lymphocytes metabolism
CD8-Positive T-Lymphocytes metabolism
Cytokines metabolism
Female
Humans
Immune System metabolism
Interferon-gamma metabolism
Interleukin-2 metabolism
Kinetics
L-Selectin biosynthesis
Leukocyte Common Antigens biosynthesis
Lymphocytes metabolism
Male
Middle Aged
Neutrophils metabolism
T-Lymphocytes immunology
Time Factors
Transplantation Conditioning
Tumor Necrosis Factor Receptor Superfamily, Member 7 biosynthesis
fas Receptor biosynthesis
Multiple Sclerosis blood
Multiple Sclerosis therapy
Stem Cell Transplantation methods
T-Lymphocytes metabolism
Transplantation, Autologous methods
Subjects
Details
- Language :
- English
- ISSN :
- 0268-3369
- Volume :
- 37
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Bone marrow transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 16518423
- Full Text :
- https://doi.org/10.1038/sj.bmt.1705333