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The selective inhibition of inducible nitric oxide synthase prevents intestinal ischemia-reperfusion injury in mice.
- Source :
-
Nitric oxide : biology and chemistry [Nitric Oxide] 2006 May; Vol. 14 (3), pp. 212-8. Date of Electronic Publication: 2006 Feb 28. - Publication Year :
- 2006
-
Abstract
- Nitric oxide (NO) involvement in intestinal ischemia-reperfusion (I/R) injury has been widely suggested but its protective or detrimental role remains still question of debate. Here, we examine the impact of supplementation or inhibition of NO availability on intestinal dysmotility and inflammation caused by mesenteric I/R in mice. Ischemia 45min and reperfusion 24h were performed by superior mesenteric artery occlusion in female Swiss mice. Saline-treated sham-operated (S) or normal mice without surgery (N) served as controls. Drugs were subcutaneously injected 0, 4, 8, and 18 h after ischemia. Upper gastrointestinal transit (GIT, estimated through black marker gavage), intestinal myeloperoxidase activity (MPO), intestinal malondialdehyde levels (MDA), Evans blue extravasation (EB), intestinal histological damage, and mean arterial pressure (MAP) were considered. In I/R mice, GIT was significantly delayed compared to S and N groups; MPO activity and EB extravasation enhanced, whereas MDA levels did not change. Compared to N and S groups, in I/R mice selective iNOS inhibitor P-BIT significantly prevented motor, MPO and EB changes; putative iNOS inhibitor aminoguanidine significantly counteracted GIT delay but not neutrophil recruitment and the increase in vascular permeability; NOS inhibitor l-NAME and NO precursor l-arginine were scarcely or no effective. Furthermore, in S mice aminoguanidine caused a significant increase of MPO activity reverted by H(1) histamine receptor antagonist pre-treatment. Unlike P-BIT, aminoguanidine and l-NAME injection increased MAP. These findings confirm a detrimental role for iNOS-derived NO overproduction during reperfusion. Aminoguanidine-associated neutrophil recruitment suggests that this drug could act through mechanisms additional to iNOS inhibition involving both eNOS blockade, as indicated by its hemodynamic effects, and indirect activation of H(1) histamine receptors.
- Subjects :
- Animals
Blood Pressure drug effects
Enzyme Inhibitors pharmacology
Female
Gastrointestinal Transit drug effects
Guanidines pharmacology
Intestine, Small pathology
Malondialdehyde analysis
Mice
Mice, Inbred Strains
NG-Nitroarginine Methyl Ester pharmacology
Peroxidase analysis
Thiourea analogs & derivatives
Thiourea pharmacology
Intestine, Small blood supply
Intestine, Small enzymology
Nitric Oxide Synthase Type II antagonists & inhibitors
Reperfusion Injury prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8603
- Volume :
- 14
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nitric oxide : biology and chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16504557
- Full Text :
- https://doi.org/10.1016/j.niox.2005.11.006