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DNA vaccines based on genetically detoxified derivatives of pneumolysin fail to protect mice against challenge with Streptococcus pneumoniae.
- Source :
-
FEMS immunology and medical microbiology [FEMS Immunol Med Microbiol] 2006 Mar; Vol. 46 (2), pp. 291-7. - Publication Year :
- 2006
-
Abstract
- The 7-valent polysaccharide conjugate vaccine currently administered against Streptococcus pneumoniae has been shown to be highly effective in high risk-groups, but its use in developing countries will probably not be possible due to high costs. The use of conserved protein antigens using the genetic vaccination strategy is an interesting alternative for the development of a cost-effective vaccine. We have analyzed the potential of DNA vaccines expressing genetically detoxified derivatives of pneumolysin (pneumolysoids) against pneumococcal infections, and compared this with immunization using recombinant protein. The purified recombinant pneumolysoid with the highest residual cytolytic activity was able to confer partial protection against a lethal intraperitoneal challenge, with the induction of high antibody levels. Immunization with DNA vaccines expressing pneumolysoids, on the other hand, induced a significantly lower antibody response and no protection was observed.
- Subjects :
- Animals
Bacterial Proteins genetics
Bacterial Proteins immunology
Bacterial Proteins toxicity
Cell Line
Cricetinae
Humans
Mice
Mice, Inbred BALB C
Pneumococcal Vaccines genetics
Recombinant Proteins immunology
Streptococcus pneumoniae genetics
Streptococcus pneumoniae immunology
Streptolysins immunology
Streptolysins toxicity
Vaccines, Conjugate administration & dosage
Vaccines, Conjugate genetics
Antibodies, Bacterial blood
Pneumococcal Infections prevention & control
Pneumococcal Vaccines administration & dosage
Streptococcus pneumoniae pathogenicity
Streptolysins genetics
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0928-8244
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS immunology and medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16487311
- Full Text :
- https://doi.org/10.1111/j.1574-695X.2006.00040.x