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Alterations in burst firing of thalamic VPL neurons and reversal by Na(v)1.3 antisense after spinal cord injury.
- Source :
-
Journal of neurophysiology [J Neurophysiol] 2006 Jun; Vol. 95 (6), pp. 3343-52. Date of Electronic Publication: 2006 Feb 15. - Publication Year :
- 2006
-
Abstract
- We recently showed that spinal cord contusion injury (SCI) at the thoracic level induces pain-related behaviors and increased spontaneous discharges, hyperresponsiveness to innocuous and noxious peripheral stimuli, and enlarged receptive fields in neurons in the ventral posterolateral (VPL) nucleus of the thalamus. These changes are linked to the abnormal expression of Na(v)1.3, a rapidly repriming voltage-gated sodium channel. In this study, we examined the burst firing properties of VPL neurons after SCI. Adult male Sprague-Dawley rats underwent contusion SCI at the T9 level. Four weeks later, when Na(v)1.3 protein was upregulated within VPL neurons, extracellular unit recordings were made from VPL neurons in intact animals, those with SCI, and in SCI animals after receiving lumbar intrathecal injections of Na(v)1.3 antisense or mismatch oligodeoxynucleotides for 4 days. After SCI, VPL neurons with identifiable peripheral receptive fields showed rhythmic oscillatory burst firing with changes in discrete burst properties, and alternated among single-spike, burst, silent, and spindle wave firing modes. Na(v)1.3 antisense, but not mismatch, partially reversed alterations in burst firing after SCI. These results demonstrate several newly characterized changes in spontaneous burst firing properties of VPL neurons after SCI and suggest that abnormal expression of Na(v)1.3 contributes to these phenomena.
- Subjects :
- Adaptation, Physiological
Animals
DNA, Antisense genetics
Gene Silencing
Male
NAV1.3 Voltage-Gated Sodium Channel
Nerve Tissue Proteins genetics
Neuronal Plasticity
Rats
Rats, Sprague-Dawley
Sodium Channels genetics
Action Potentials
Biological Clocks
Nerve Tissue Proteins metabolism
Neurons
Sodium Channels metabolism
Spinal Cord Injuries physiopathology
Ventral Thalamic Nuclei physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3077
- Volume :
- 95
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of neurophysiology
- Publication Type :
- Academic Journal
- Accession number :
- 16481457
- Full Text :
- https://doi.org/10.1152/jn.01009.2005