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B cells play an important role in lipopolysaccharide-induced bone resorption.
- Source :
-
Calcified tissue international [Calcif Tissue Int] 2006 Mar; Vol. 78 (3), pp. 125-32. Date of Electronic Publication: 2006 Feb 06. - Publication Year :
- 2006
-
Abstract
- The host immune system, especially activated T cells, plays a crucial role in inflammatory bone resorption and osteoclastogenesis. Previously, we showed that T cells are involved in inflammatory bone resorption in vivo. However, little is known about whether B cells are involved in inflammatory bone resorption and how B cells take part in osteoclastogenesis. Therefore, the aim of this study was to examine whether B c ells truly influence inflammatory bone resorption in vivo. Alveolar bone resorption in normal mice, in SCID mice that lack both B and T cells, and in B cell-reconstituted SCID mice was compared histopathologically after repeated injections of lipopolysaccharide (LPS) into the gingiva. Furthermore, we examined whether the B cells that are stimulated by LPS are involved in osteoclastogenesis in vitro. As a result, the B cell-reconstituted SCID mice showed stronger inflammatory bone resorption than the SCID mice. Also, in vitro, LPS-stimulated B cells enhanced osteoclastogenesis and anti-tumor necrosis factor (TNF)-alpha antibody completely blocked osteoclastogenesis induced by LPS-stimulated B cells. These results suggest that B cells promote inflammatory bone resorption through TNF-alpha.
- Subjects :
- Animals
B-Lymphocytes cytology
B-Lymphocytes drug effects
Bone Marrow Cells cytology
Bone Resorption pathology
Cells, Cultured
Femur cytology
Flow Cytometry
Gingiva drug effects
Gingiva pathology
Immunohistochemistry
Lymphocyte Activation
Male
Mice
Mice, Inbred Strains
Mice, SCID
Spleen cytology
Tibia cytology
Tumor Necrosis Factor-alpha metabolism
B-Lymphocytes metabolism
Bone Resorption chemically induced
Bone Resorption immunology
Lipopolysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0171-967X
- Volume :
- 78
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Calcified tissue international
- Publication Type :
- Academic Journal
- Accession number :
- 16467977
- Full Text :
- https://doi.org/10.1007/s00223-005-0149-x