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Lead inhibited N-methyl-D-aspartate receptor-independent long-term potentiation involved ryanodine-sensitive calcium stores in rat hippocampal area CA1.
- Source :
-
Neuroscience [Neuroscience] 2006 May 12; Vol. 139 (2), pp. 463-73. Date of Electronic Publication: 2006 Feb 02. - Publication Year :
- 2006
-
Abstract
- Lead exposure is known to be associated with cognitive dysfunction in children. Impairment of the induction of long-term potentiation (LTP) has been reported in area CA1 of rat hippocampus following lead exposure in vivo and in vitro. The present study was carried out to investigate whether the alterations of N-methyl-d-aspartate (NMDA) receptor-independent LTP following lead exposure involve internal calcium stores in hippocampus CA1 synapses. Monosynaptic field excitatory postsynaptic potentials in hippocampal slice area CA1 were recorded using the whole-cell patch-clamp upon acute lead treatment, and these studies were coupled with calcium imaging experiments to observe internal calcium changes in cultured hippocampal neurons. Inhibiting calcium release by ryanodine significantly reduced NMDA receptor-independent LTP, and depletion of internal calcium stores with thapsigargin blocked this form of LTP. Caffeine, an agonist of ryanodine receptors, enhanced this form of LTP. However, caffeine-enhanced NMDA receptor-independent LTP was depressed after bath application of lead. Moreover, lead further decreased ryanodine- and thapsigargin-reduced NMDA receptor-independent LTP. Calcium imaging also confirmed that lead had an effect on internal calcium release and uptake. Taken together, these results demonstrated that lead inhibited NMDA receptor-independent LTP by action on calcium release and uptake by ryanodine-sensitive stores in rat hippocampal area CA1.
- Subjects :
- Aniline Compounds
Animals
Animals, Newborn
Caffeine pharmacology
Cells, Cultured
Diagnostic Imaging methods
Drug Interactions
Electric Stimulation methods
Embryo, Mammalian
Enzyme Inhibitors pharmacology
In Vitro Techniques
Long-Term Potentiation physiology
Long-Term Potentiation radiation effects
Patch-Clamp Techniques methods
Rats
Receptors, N-Methyl-D-Aspartate physiology
Ryanodine agonists
Thapsigargin pharmacology
Xanthenes
Calcium metabolism
Hippocampus cytology
Lead pharmacology
Long-Term Potentiation drug effects
Neurons drug effects
Ryanodine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 139
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 16457957
- Full Text :
- https://doi.org/10.1016/j.neuroscience.2005.12.033