High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

Authors :: Aires-de-Sousa M
Boye K
de Lencastre H
Deplano A
Enright MC
Etienne J
Friedrich A
Harmsen D
Holmes A
Huijsdens XW
Kearns AM
Mellmann A
Meugnier H
Rasheed JK
Spalburg E
Strommenger B
Struelens MJ
Tenover FC
Thomas J
Vogel U
Westh H
Xu J
Witte W
Source :: Journal of clinical microbiology [J Clin Microbiol] 2006 Feb; Vol. 44 (2), pp. 619-21.
Publication Year :: 2006
Abstract: Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

Subjects :: DNA, Bacterial analysis
Humans
Laboratories
Observer Variation
Reproducibility of Results
Software
Staphylococcal Infections microbiology
Staphylococcus aureus genetics
Bacterial Typing Techniques methods
Bacterial Typing Techniques statistics & numerical data
Sequence Analysis, DNA methods
Sequence Analysis, DNA statistics & numerical data
Staphylococcal Protein A genetics
Staphylococcus aureus classification

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