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A pilot study investigating a novel subcutaneously implanted pre-cellularised scaffold for tissue engineering of intestinal mucosa.
- Source :
-
European cells & materials [Eur Cell Mater] 2006 Jan 31; Vol. 11, pp. 27-33; discussion 34. Date of Electronic Publication: 2006 Jan 31. - Publication Year :
- 2006
-
Abstract
- Tissue engineering of the small intestine offers an alternative to long-term intravenous nutrition and transplantation in patients with intestinal failure. Initial work, although encouraging, is limited by the volume of neonatal tissue required to produce a small neomucosal cyst. Our novel approach is to implant tubular poly-lactide-co-glycolide (PGLA) foam scaffolds subcutaneously. The aim of this study was to investigate whether these scaffolds would support growth of intestinal neomucosa. PGLA scaffolds were implanted subcutaneously into 8 Lewis rats; after 5 weeks, 'organoid units' were injected into the lumens. Tissue was assessed histologically after harvesting and quantitative immunohistochemistry was performed using antibodies against vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGF-R2), fibroblast growth factor basic (bFGF) and fibroblast growth factor receptor 2 (FGF-R2). At 4 weeks post organoid unit implantation, clearly recognisable mucosa and submucosa was present on the luminal surface of the scaffold. Densities of VEGF and VEGF-R2 positive cells increased with time post organoid unit implantation. This pilot study demonstrates that it is possible to tissue engineer small intestinal neomucosa using subcutaneously implanted PLGA scaffolds. The yield of the process compares favourably to the published literature. Further work is required to optimise the technique.
- Subjects :
- Animals
Cell Proliferation
Fibroblast Growth Factor 2 analysis
Fibroblast Growth Factor 2 physiology
Immunohistochemistry
Intestinal Mucosa chemistry
Male
Materials Testing
Neovascularization, Physiologic
Organoids cytology
Organoids growth & development
Organoids physiology
Pilot Projects
Polylactic Acid-Polyglycolic Acid Copolymer
Prostheses and Implants
Rats
Rats, Inbred Lew
Receptor, Fibroblast Growth Factor, Type 2 analysis
Receptor, Fibroblast Growth Factor, Type 2 physiology
Receptors, Vascular Endothelial Growth Factor analysis
Receptors, Vascular Endothelial Growth Factor physiology
Vascular Endothelial Growth Factor A analysis
Vascular Endothelial Growth Factor A physiology
Implants, Experimental
Intestinal Mucosa cytology
Intestinal Mucosa growth & development
Lactic Acid
Polyglycolic Acid
Polymers
Tissue Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1473-2262
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- European cells & materials
- Publication Type :
- Academic Journal
- Accession number :
- 16447140
- Full Text :
- https://doi.org/10.22203/ecm.v011a04