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Red pool chlorophylls of photosystem I of the cyanobacterium Thermosynechococcus elongatus: a single-molecule study.

Authors :
Elli AF
Jelezko F
Tietz C
Studier H
Brecht M
Bittl R
Wrachtrup J
Source :
Biochemistry [Biochemistry] 2006 Feb 07; Vol. 45 (5), pp. 1454-8.
Publication Year :
2006

Abstract

Photosystem I reaction centers of the cyanobacterium Thermosynechococcus elongatus have been investigated using single-molecule spectroscopy. Single-molecule fluorescence emission spectra reveal a new fluorescence band located at 745 nm. Fluorescence polarization spectroscopy and fluorescence autocorrelation analysis show that only a few chlorophylls are responsible for the photoemission from the Photosystem I trimer at low temperature. Intersystem crossing parameters of the red pool chlorophylls have been determined via fluorescence autocorrelation measurements. The triplet yield of the red chlorophylls is strongly reduced in comparison to chlorophyll a in solution. Strong quenching of the triplet state indicates that the red chlorophylls are located in close contact to carotenoids.

Details

Language :
English
ISSN :
0006-2960
Volume :
45
Issue :
5
Database :
MEDLINE
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
16445287
Full Text :
https://doi.org/10.1021/bi0521700