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Oregano (Lippia spp.) kills Giardia intestinalis trophozoites in vitro: antigiardiasic activity and ultrastructural damage.
- Source :
-
Parasitology research [Parasitol Res] 2006 May; Vol. 98 (6), pp. 557-60. Date of Electronic Publication: 2006 Jan 20. - Publication Year :
- 2006
-
Abstract
- In the world, giardiosis is still a very important parasitic disease; only in Asia, Africa and America, there are more than 200 million of infected people in a year. The usual treatments are drugs that produce undesirable secondary effects, perhaps favouring the resistant strain selection. One alternative is to research compounds from plants used as antidiarrhoeic or antiparasitic in the traditional medicine. In a previous work, we found that Lippia beriandieri (Oregano) revealed to be more potent than tinidazole, a common antigiardiasic drug. In this current work, we tested the cell viability by re-culture and reduction of MTT-tetrazolium salts to MTT-formazan, and we showed the effect of oregano ethanolic extracts against Giardia intestinalis (synonyms: Giardia duodenalis, Giardia lamblia) trophozoites at concentrations ranging form 58 to 588 microg. We demonstrated the ultrastructural injury produced by oregano extracts in this parasite. Trophozoites lost their size and shape and showed damage in nucleus structure, perhaps by breaking the pattern of nucleoskeleton proteins.
- Subjects :
- Animals
Cell Nucleus ultrastructure
Cell Survival
Humans
Microscopy, Electron, Transmission
Staining and Labeling
Tetrazolium Salts metabolism
Thiazoles metabolism
Antiprotozoal Agents pharmacology
Giardia lamblia drug effects
Giardia lamblia ultrastructure
Origanum chemistry
Plant Extracts pharmacology
Trophozoites drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0932-0113
- Volume :
- 98
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 16425064
- Full Text :
- https://doi.org/10.1007/s00436-005-0082-y