Back to Search
Start Over
Direct costimulatory effect of TLR3 ligand poly(I:C) on human gamma delta T lymphocytes.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Feb 01; Vol. 176 (3), pp. 1348-54. - Publication Year :
- 2006
-
Abstract
- TLR3 recognizes viral dsRNA and its synthetic mimetic polyinosinic-polycytidylic acid (poly(I:C)). TLR3 expression is commonly considered to be restricted to dendritic cells, NK cells, and fibroblasts. In this study we report that human gammadelta and alphabeta T lymphocytes also express TLR3, as shown by quantitative real-time PCR, flow cytometry, and confocal microscopy. Although T cells did not respond directly to poly(I:C), we observed a dramatic increase in IFN-gamma secretion and an up-regulation of CD69 when freshly isolated gammadelta T cells were stimulated via TCR in the presence of poly(I:C) without APC. IFN-gamma secretion was partially inhibited by anti-TLR3 Abs. In contrast, poly(I:C) did not costimulate IFN-gamma secretion by alphabeta T cells. These results indicate that TLR3 signaling is differentially regulated in TCR-stimulated gammadelta and alphabeta T cells, suggesting an early activation of gammadelta T cells in antiviral immunity.
- Subjects :
- Cell Line
Cell Proliferation drug effects
Cells, Cultured
Clone Cells
Humans
Interferon Inducers metabolism
Interferon-gamma biosynthesis
Ligands
Lymphocyte Activation immunology
Poly I-C metabolism
Receptors, Antigen, T-Cell, alpha-beta biosynthesis
Receptors, Antigen, T-Cell, alpha-beta metabolism
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets metabolism
Toll-Like Receptor 3 biosynthesis
Interferon Inducers pharmacology
Lymphocyte Activation drug effects
Poly I-C pharmacology
Receptors, Antigen, T-Cell, gamma-delta metabolism
T-Lymphocyte Subsets drug effects
Toll-Like Receptor 3 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 176
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 16424161
- Full Text :
- https://doi.org/10.4049/jimmunol.176.3.1348