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Accelerated lipid absorption in mice overexpressing intestinal SR-BI.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2006 Mar 17; Vol. 281 (11), pp. 7214-9. Date of Electronic Publication: 2006 Jan 18. - Publication Year :
- 2006
-
Abstract
- Dietary cholesterol absorption contributes to a large part of the circulating cholesterol. However, the mechanism of sterol intestinal uptake is not clearly elucidated. Scavenger receptor class B type I (SR-BI), major component in the control of cholesterol homeostasis, is expressed in the intestine, but its role in this organ remains unclear. We have generated transgenic mice overexpressing SR-BI primarily in the intestine by using the mouse SR-BI gene under the control of intestinal specific "apoC-III enhancer coupled with apoA-IV promoter." We found SR-BI overexpression with respect to the natural protein along the intestine and at the top of the villosities. After a meal containing [(14)C]cholesterol and [(3)H]triolein, SR-BI transgenic mice presented a rise in intestinal absorption of both lipids that was not due to a defect in chylomicron clearance nor to a change in the bile flow or the bile acid content. Nevertheless, SR-BI transgenic mice showed a decrease of total cholesterol but an increase of triglyceride content in plasma without any change in the high density lipoprotein apoA-I level. Thus, we described for the first time a functional role in vivo for SR-BI in cholesterol but also in triglyceride intestinal absorption.
- Subjects :
- Absorption
Animals
Apolipoproteins chemistry
Bile Acids and Salts metabolism
Cell Membrane metabolism
Cholesterol chemistry
Cholesterol metabolism
Chylomicrons chemistry
DNA, Complementary metabolism
Homeostasis
Immunohistochemistry
Intestines chemistry
Lipoproteins chemistry
Liver metabolism
Mice
Mice, Transgenic
Promoter Regions, Genetic
Receptors, Lipoprotein metabolism
Receptors, Scavenger chemistry
Tissue Distribution
Triglycerides metabolism
Triolein chemistry
Intestinal Mucosa metabolism
Lipids chemistry
Scavenger Receptors, Class B metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 281
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16421100
- Full Text :
- https://doi.org/10.1074/jbc.M508868200