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Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NF kappa B.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2005 Oct 28; Vol. 11 (40), pp. 6258-61. - Publication Year :
- 2005
-
Abstract
- Aim: To investigate the reduction of cell viability in human hepatocellular carcinoma (HCC) cell lines induced by inhibition of nuclear factor kappa B (NF kappa B).<br />Methods: HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family, Fas ligand, TNF alpha and TNF-related apoptosis-inducing ligand (TRAIL) at the point of the reduction of cell viability by inhibiting NF kappa B.<br />Results: E3330 decreased NF kappa B levels in HLE cells stimulated by TNF and TRAIL. The cytotoxicity of the combination of TRAIL, TNF alpha, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. However, the combination of some chemotherapeutic drugs and E3330 did not decrease the cell viability.<br />Conclusion: Inhibition of NF kappa B sensitizes human HCC cell lines to TNF-mediated apoptosis including TRAIL, and TRAIL-based tumor therapy might be a powerful potential therapeutic tool in the treatment of human HCC.
- Subjects :
- Apoptosis Regulatory Proteins metabolism
Cell Line, Tumor
Cell Proliferation
Cell Survival
Genes, Reporter
Humans
Membrane Glycoproteins metabolism
NF-kappa B metabolism
TNF-Related Apoptosis-Inducing Ligand
Apoptosis physiology
Benzoquinones pharmacology
Carcinoma, Hepatocellular metabolism
Liver Neoplasms metabolism
NF-kappa B antagonists & inhibitors
Propionates pharmacology
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 11
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 16419152
- Full Text :
- https://doi.org/10.3748/wjg.v11.i40.6258