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Differential expression of espin isoforms during epithelial morphogenesis, stereociliogenesis and postnatal maturation in the developing inner ear.
- Source :
-
Developmental biology [Dev Biol] 2006 Mar 01; Vol. 291 (1), pp. 83-95. Date of Electronic Publication: 2006 Jan 17. - Publication Year :
- 2006
-
Abstract
- The espins are a family of multifunctional actin cytoskeletal proteins. They are present in hair cell stereocilia and are the target of mutations that cause deafness and vestibular dysfunction. Here, we demonstrate that the different espin isoforms are expressed in complex spatiotemporal patterns during inner ear development. Espin 3 isoforms were prevalent in the epithelium of the otic pit, otocyst and membranous labyrinth as they underwent morphogenesis. This espin was down-regulated ahead of hair cell differentiation and during neuroblast delamination. Espin also accumulated in the epithelium of branchial clefts and pharyngeal pouches and during branching morphogenesis in other embryonic epithelial tissues, suggesting general roles for espins in epithelial morphogenesis. Espin reappeared later in inner ear development in differentiating hair cells. Its levels and compartmentalization to stereocilia increased during the formation and maturation of stereociliary bundles. Late in embryonic development, espin was also present in a tail-like process that emanated from the hair cell base. Increases in the levels of espin 1 and espin 4 isoforms correlated with stereocilium elongation and maturation in the vestibular system and cochlea, respectively. Our results suggest that the different espin isoforms play specific roles in actin cytoskeletal regulation during epithelial morphogenesis and hair cell differentiation.
- Subjects :
- Animals
Animals, Newborn
Cell Differentiation
Cochlea embryology
Cochlea growth & development
Cochlea metabolism
Cytoskeleton metabolism
Ear, Inner metabolism
Epithelium embryology
Epithelium growth & development
Epithelium metabolism
Hair Cells, Auditory metabolism
Lacrimal Apparatus embryology
Lacrimal Apparatus growth & development
Lacrimal Apparatus metabolism
Lung embryology
Lung growth & development
Lung metabolism
Mice
Morphogenesis
Protein Isoforms metabolism
Rats
Rats, Sprague-Dawley
Vestibule, Labyrinth embryology
Vestibule, Labyrinth growth & development
Vestibule, Labyrinth metabolism
Ear, Inner embryology
Ear, Inner growth & development
Hair Cells, Auditory embryology
Hair Cells, Auditory growth & development
Microfilament Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0012-1606
- Volume :
- 291
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 16413524
- Full Text :
- https://doi.org/10.1016/j.ydbio.2005.12.021