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WARP is a novel multimeric component of the chondrocyte pericellular matrix that interacts with perlecan.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2006 Mar 17; Vol. 281 (11), pp. 7341-9. Date of Electronic Publication: 2006 Jan 06. - Publication Year :
- 2006
-
Abstract
- WARP is a novel member of the von Willebrand factor A domain superfamily of extracellular matrix proteins that is expressed by chondrocytes. WARP is restricted to the presumptive articular cartilage zone prior to joint cavitation and to the articular cartilage and fibrocartilaginous elements in the joint, spine, and sternum during mouse embryonic development. In mature articular cartilage, WARP is highly specific for the chondrocyte pericellular microenvironment and co-localizes with perlecan, a prominent component of the chondrocyte pericellular region. WARP is present in the guanidine-soluble fraction of cartilage matrix extracts as a disulfide-bonded multimer, indicating that WARP is a strongly interacting component of the cartilage matrix. To investigate how WARP is integrated with the pericellular environment, we studied WARP binding to mouse perlecan using solid phase and surface plasmon resonance analysis. WARP interacts with domain III-2 of the perlecan core protein and the heparan sulfate chains of the perlecan domain I with K(D) values in the low nanomolar range. We conclude that WARP forms macromolecular structures that interact with perlecan to contribute to the assembly and/or maintenance of "permanent" cartilage structures during development and in mature cartilages.
- Subjects :
- Amino Acid Motifs
Animals
Cartilage metabolism
Cartilage, Articular metabolism
Cell Line
DNA, Complementary metabolism
Disulfides chemistry
Dose-Response Relationship, Drug
Extracellular Matrix Proteins chemistry
Humans
Immunohistochemistry
Immunoprecipitation
In Situ Hybridization
Kinetics
Mice
Protein Binding
Protein Structure, Tertiary
RNA, Messenger metabolism
Recombinant Proteins chemistry
Surface Plasmon Resonance
Time Factors
Tissue Distribution
von Willebrand Factor chemistry
Chondrocytes metabolism
Extracellular Matrix metabolism
Extracellular Matrix Proteins physiology
Heparan Sulfate Proteoglycans chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 281
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16407285
- Full Text :
- https://doi.org/10.1074/jbc.M513746200