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Strain-dependent suppressive effects of BCG vaccination on asthmatic reactions in BALB/c mice.
- Source :
-
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology [Ann Allergy Asthma Immunol] 2005 Dec; Vol. 95 (6), pp. 571-8. - Publication Year :
- 2005
-
Abstract
- Background: The choice of BCG vaccine strain may play an important role in vaccination efficiency.<br />Objective: To investigate whether the suppressive effects of BCG on asthma depend on the strain of BCG.<br />Methods: Female BALB/c mice were injected intraperitoneally with 1 of 4 different strains of BCG (1 X 10(6) CFU): Pasteur F1173P2, Tokyo 172, Tice, and Connaught. Seven days later, the animals were sensitized by 2 injections of ovalbumin (20 microg) at 2-week intervals before being provoked with 1% ovalbumin aerosols on 3 successive days. Thereafter, the mice underwent a methacholine bronchial challenge and were killed to quantify the inflammatory cells and cytokines in bronchoalveolar lavage fluid and the supernatant of cultured splenocytes.<br />Results: The eosinophil proportion in the bronchoalveolar lavage fluid was significantly lower and the concentration of interferon-gamma and the interferon-gamma-interleukin 5 (IL-5) ratio in the supernatant of cultured splenocytes were significantly higher in each of the BCG-treated groups (n=10 per group) than in the asthma control group (n=15). However, the methacholine sensitivity (P < .05) and IL-5 concentration (P < .01) in the supernatant of cultured splenocytes were significantly lower only in the group treated with the Tokyo strain of BCG. There was a significant positive correlation between IL-5 and IL-10 concentrations (r = 0.79; P < .001).<br />Conclusions: The 4 strains of BCG suppressed asthma to different degrees, but all strains induced a shift in the T(H)1/T(H)2 balance toward T(H)1 without increasing IL-10-related regulatory T-cell activity.
- Subjects :
- Animals
BCG Vaccine pharmacology
Bronchoalveolar Lavage Fluid cytology
Bronchoalveolar Lavage Fluid immunology
Cytokines metabolism
Female
Humans
Immunoglobulin E blood
Mice
Mice, Inbred BALB C
Ovalbumin immunology
Respiratory Function Tests
Species Specificity
Specific Pathogen-Free Organisms
Vaccination
Asthma immunology
BCG Vaccine administration & dosage
Bronchial Hyperreactivity immunology
Mycobacterium bovis classification
Subjects
Details
- Language :
- English
- ISSN :
- 1081-1206
- Volume :
- 95
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16400898
- Full Text :
- https://doi.org/10.1016/S1081-1206(10)61021-6