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Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus.
- Source :
-
Virus research [Virus Res] 2006 Jun; Vol. 118 (1-2), pp. 98-104. Date of Electronic Publication: 2005 Dec 27. - Publication Year :
- 2006
-
Abstract
- The function of the glycosylated protein 3 (GP3), a porcine reproductive and respiratory syndrome virus (PRRSV) associated protein is poorly known. In the present study, the gene encoding GP3 (ORF3), lacking the highly hydrophobic domain in the N- and C-termini was expressed as GST-fusion proteins in E. coli. Monoclonal antibodies (MAbs) against GP3 were developed and used to probe a series of GP3 peptides using ELISA. After precise analysis by sequential deletion of the terminal amino acid residues from each peptide, the minimal epitopes recognized by the MAbs were localized to W(74)CRIGHDRCGED(85) and Y(67)EPGRSLW(74). The epitope sequences were well conserved among most of the North American-type isolates, with the exception of two amino acid mutations in both epitopes in a few of these isolates. Mutational analysis revealed that these mutants were not recognized by any of the five MAbs, indicating that genetic variation could lead to altered antigenicity. Eight out of nine peptide fragments, 58-72aa, 73-87aa, 88-101aa, 102-115aa, 50-65aa, 66-81aa, 80-95aa and 94-109aa were recognized by PRRSV-positive pig serum as determined by Western blot analysis. The results herein may elucidate partially the antigenic structure of GP3 and variations of PRRSV.
- Subjects :
- Amino Acid Sequence
Animals
Antibodies, Monoclonal immunology
Antibodies, Viral blood
Antigens, Viral genetics
Blotting, Western
Cell Line
Conserved Sequence
Epitopes, B-Lymphocyte immunology
Glycoproteins genetics
Glycoproteins immunology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mutation
Porcine Reproductive and Respiratory Syndrome immunology
Porcine respiratory and reproductive syndrome virus genetics
Swine
Viral Proteins genetics
Antigens, Viral immunology
Epitope Mapping
Porcine respiratory and reproductive syndrome virus immunology
Viral Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1702
- Volume :
- 118
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 16384621
- Full Text :
- https://doi.org/10.1016/j.virusres.2005.11.019