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G protein activation is prerequisite for functional coupling between Galpha/Gbetagamma and tubulin/microtubules.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2006 Feb 10; Vol. 340 (2), pp. 441-8. Date of Electronic Publication: 2005 Dec 15. - Publication Year :
- 2006
-
Abstract
- Heterotrimeric G proteins participate in signal transduction by transferring signals from cell surface receptors to intracellular effector molecules. Interestingly, recent results suggest that G proteins also interact with microtubules and participate in cell division and differentiation. It has been shown earlier that both alpha and betagamma subunits of G proteins modulate microtubule assembly in vitro. Since G protein activation and subsequent dissociation of alpha and betagamma subunits are necessary for G proteins to participate in signaling processes, here we asked if similar activation is required for modulation of microtubule assembly by G proteins. We reconstituted Galphabetagamma heterotrimer from myristoylated-Galpha and prenylated-Gbetagamma, and found that the heterotrimer blocks Gi1alpha activation of tubulin GTPase and inhibits the ability of Gbeta1gamma2 to promote in vitro microtubule assembly. Results suggest that G protein activation is required for functional coupling between Galpha/Gbetagamma and tubulin/microtubules, and supports the notion that regulation of microtubules is an integral component of G protein mediated signaling.
- Subjects :
- Animals
Brain metabolism
GTP-Binding Protein alpha Subunits antagonists & inhibitors
GTP-Binding Protein alpha Subunits physiology
GTP-Binding Protein beta Subunits antagonists & inhibitors
GTP-Binding Protein beta Subunits physiology
GTP-Binding Protein gamma Subunits antagonists & inhibitors
GTP-Binding Protein gamma Subunits physiology
Microtubules ultrastructure
Protein Structure, Quaternary
Sheep
GTP-Binding Protein alpha Subunits metabolism
GTP-Binding Protein beta Subunits metabolism
GTP-Binding Protein gamma Subunits metabolism
Microtubules metabolism
Tubulin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 340
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 16380086
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.12.026