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CD5+ B cells are preferentially expanded in rabbit appendix: the role of CD5 in B cell development and selection.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2006; Vol. 30 (8), pp. 711-22. Date of Electronic Publication: 2005 Nov 02. - Publication Year :
- 2006
-
Abstract
- Although only a small proportion of mouse and human B cells are CD5(+), most adult rabbit B cells express CD5. However, CD5 was not detectable on the majority of B cells in neonatal appendix 1 and 3days after birth. Cell trafficking studies demonstrated that CD5(+) and CD5(-) CD62L(+) B cells from bone marrow migrated into appendix. There, CD5(+) B cells were preferentially expanded and predominated by approximately 2weeks of age. In mutant ali/ali rabbits, VHa2(+) B cells develop through gene conversion-like alteration of rearranged VH genes upstream of deleted VH1a2. Correlated appearance of individual CD5(+) germinal centers and VHa2(+) B-cells in mutant appendix suggests that CD5 binding positively selects cells with a2(+) framework regions that bind CD5. Following negative and positive selection, cells with diversified rearranged heavy- and light-chain sequences exit appendix, migrate to peripheral tissues and constitute the preimmune repertoire of CD5(+) B cells that encounter foreign antigens.
- Subjects :
- Amino Acid Sequence
Animals
Appendix cytology
B-Lymphocytes cytology
Base Sequence
Bone Marrow immunology
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Genes, Immunoglobulin
Immunoglobulin Heavy Chains genetics
Immunoglobulin M
Immunoglobulin Variable Region genetics
L-Selectin
Molecular Sequence Data
Rabbits growth & development
Spleen cytology
Spleen immunology
Appendix immunology
B-Lymphocytes immunology
CD5 Antigens physiology
Rabbits immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0145-305X
- Volume :
- 30
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16375969
- Full Text :
- https://doi.org/10.1016/j.dci.2005.10.001