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Silencing the hsp25 gene eliminates migration capability of the highly metastatic murine 4T1 breast adenocarcinoma cell.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2006; Vol. 27 (1), pp. 17-26. Date of Electronic Publication: 2005 Dec 08. - Publication Year :
- 2006
-
Abstract
- The 25-kDa heat shock protein (Hsp25) is associated with various malignancies and is expressed at high levels in biopsies as well as circulating in the serum of breast cancer patients. In this study, we used RNA interference technology to silence the hsp25 gene in 4T1 breast adenocarcinoma cells, known as a poorly immunogenic, highly metastatic cell line. We demonstrate that transfection of 4T1 cells with short interference RNA-Hsp25 dramatically inhibits proliferation as compared with control transfected cells. In addition, we show that 4T1 cells transfected with short interference RNA-Hsp25 abrogates tumor migration potential by a mechanism that is in part due to the repression of matrix metalloproteinase 9 expression and a concomitant upregulation of its antagonist, tissue inhibitor metalloproteinase 1. Taken together, these findings provide a model system for the study of metastatic potential of tumors and are suggestive of an earlier unrecognized role for Hsp25 in tumor migration.<br /> (Copyright 2006 S. Karger AG, Basel.)
- Subjects :
- Base Sequence
Cell Movement
Cell Proliferation
Female
HSP27 Heat-Shock Proteins
Heat-Shock Proteins physiology
Humans
Matrix Metalloproteinase 9 metabolism
Molecular Chaperones
Molecular Sequence Data
Neoplasm Proteins physiology
Transfection
Tumor Cells, Cultured
Adenocarcinoma genetics
Adenocarcinoma pathology
Breast Neoplasms genetics
Breast Neoplasms pathology
Heat-Shock Proteins biosynthesis
Heat-Shock Proteins genetics
Neoplasm Metastasis physiopathology
Neoplasm Proteins biosynthesis
Neoplasm Proteins genetics
RNA Interference
Subjects
Details
- Language :
- English
- ISSN :
- 1010-4283
- Volume :
- 27
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 16340246
- Full Text :
- https://doi.org/10.1159/000090152