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[Molecular epidemiology of diarrhea among children with Norwalk-like virus in a hospital of Guangzhou in autumn and winter].
- Source :
-
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi [Zhonghua Liu Xing Bing Xue Za Zhi] 2005 Jul; Vol. 26 (7), pp. 525-8. - Publication Year :
- 2005
-
Abstract
- Objective: To explore the epidemiological characteristics of Norwalk-like virus (NLVs) infection in children with diarrhea and to study the genotype and predominant cluster at a hospital in Guangzhou city.<br />Methods: Fecal specimens from 358 children with acute gastroenteritis from October 2003 to January 2004 and information about the cases were collected. NLVs was detected from the specimens by reverse transcription-polymerase chain reaction (RT-PCR) and the PCR products were purified and sequenced.<br />Results: Forty-two positive specimens were detected from the 358 fecal specimen with a positive rate of 11.73% (42/358). Of these, 40 specimens were obtained from infants younger than 3 years of age. The youngest infant infected with NLVs in this study was only 25 days. The positive rate in November (17.27%) was the highest. Eleven positive PCR products were selected and sequenced. Nucleotide sequence analysis revealed that 11 strains all belong to genogroup II (G II), and of these, 5 strains belonged to G II-3 cluster, with another 5 strains belonged to G II-4 cluster. However, one strain with its cluster could not be determined.<br />Conclusion: NLVs served as one of the important pathogens causing sporadic acute gastroenteritis among children at a hospital in Guangzhou. The predominant strains were identified as G II-3 and G II-4 cluster.
- Subjects :
- Age Distribution
Child, Preschool
China epidemiology
Feces virology
Female
Humans
Infant
Infant, Newborn
Male
Molecular Epidemiology
Norovirus classification
Norovirus physiology
Phylogeny
RNA, Viral genetics
Sequence Homology, Nucleic Acid
Sex Distribution
Caliciviridae Infections complications
Caliciviridae Infections epidemiology
Diarrhea complications
Diarrhea epidemiology
Hospitals statistics & numerical data
Norovirus genetics
Seasons
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0254-6450
- Volume :
- 26
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
- Publication Type :
- Academic Journal
- Accession number :
- 16335006