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[Study of beta-defensin-2 gene expression in the pulmonary tissue of the older rat stimulated by pneumococcal vaccine polyvalent].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2005 Nov; Vol. 36 (6), pp. 824-6. - Publication Year :
- 2005
-
Abstract
- Objective: To investigate the effect of pneumococcal vaccine polyvalent (pneumovax23) on defensin-2 gene expression in older rats.<br />Methods: Twenty older male Wistar rats (18-22 months) weighing (600 +/- 50) g were randomly allocated into two groups. The rats of experimental group were intraperitoneally injected with pneumovax23 0.2 ml/kg; the rats of control group were intraperitoneally injected with equivalent normal saline. Semiquantitive reverse transcription polymerase chain reaction (RT-PCR) was performed to detect beta-defensin-2 mRNA expression; beta-actin was used as the internal standard.<br />Results: The expression of beta-defensin-2 gene increased in the experimental group, as compared with that in the control group.<br />Conclusion: Pneumovax23 can upregulate the expression of rBD-2 gene in the lung tissue and enhance the body local immunity. These experimental data indicate that Pneumovax23 can be used to enhance beta-defensin-2 and hence protect the old rats from pneumococci.
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 36
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 16334563