Back to Search
Start Over
Peptides of human gingival crevicular fluid determined by HPLC-ESI-MS.
- Source :
-
European journal of oral sciences [Eur J Oral Sci] 2005 Dec; Vol. 113 (6), pp. 462-8. - Publication Year :
- 2005
-
Abstract
- The acidic-soluble protein content of human gingival crevicular fluid was analyzed by reverse-phase high-performance liquid chromatography (RP-HPLC), and the eluent deriving from the chromatography separation was directly introduced into an ion-trap mass spectrometer through electrospray ionization (ESI-IT MS). By this technique the molecular weight of peptides/proteins was determined with a precision of approximately 1/10,000 amu. On the basis of the chromatographic behavior and the knowledge of the molecular mass value, some peptides and proteins soluble in acidic solution were unambiguously recognized. Besides high quantities of human serum albumin, alpha-defensins 1-4 and minor amounts of cystatin A, statherin, basic PB salivary peptide and other unidentified components were detected. The presence of alpha-defensins in gingival crevicular fluid is in agreement with their relevant contribution to protein composition deriving from granulocyte secretions. Other peptides and proteins abundant in human saliva, such as proline-rich proteins (PRPs) and histatins, were not detected in gingival crevicular fluid. Further investigations will be necessary to establish the origin of statherin and PB salivary peptide in gingival crevicular fluid.
- Subjects :
- Adult
Chromatography, High Pressure Liquid
Cystatins isolation & purification
Cystatins metabolism
Female
Gingival Crevicular Fluid chemistry
Humans
Male
Middle Aged
Proteins metabolism
Reference Values
Salivary Proteins and Peptides isolation & purification
Serum Albumin isolation & purification
Serum Albumin metabolism
Spectrometry, Mass, Electrospray Ionization
alpha-Defensins isolation & purification
Gingival Crevicular Fluid metabolism
Proteins isolation & purification
Salivary Proteins and Peptides metabolism
alpha-Defensins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0909-8836
- Volume :
- 113
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- European journal of oral sciences
- Publication Type :
- Academic Journal
- Accession number :
- 16324135
- Full Text :
- https://doi.org/10.1111/j.1600-0722.2005.00246.x