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TBX1, a DiGeorge syndrome candidate gene, is inhibited by retinoic acid.
TBX1, a DiGeorge syndrome candidate gene, is inhibited by retinoic acid.
- Source :
-
The International journal of developmental biology [Int J Dev Biol] 2006; Vol. 50 (1), pp. 55-61. - Publication Year :
- 2006
-
Abstract
- Both retinoic acid (RA) and Tbx1 are definitively indispensable for the development of the pharyngeal arches. The defects produced by a loss of Tbx1 highly resemble those induced by hyper- and hypo-RA. Based on these similarities, the effects of RA on Tbx1 expression pattern were explored during pharyngeal arch development in zebrafish. Whole-mount in situ hybridization and real-time quantitative PCR were used. Zebrafish embryos were treated with 5 x 10(-8)mol/L and 10(-7)mol/L RA at 12.5 hours post fertilization for 1.5 hours, respectively. Whole-mount in situ hybridization showed that Tbx1 was expressed in the cardiac region, pharyngeal arch and otic vesicle between 24 hpf and 72 hpf in zebrafish. Tbx1 expression was obviously reduced, even lost, in the pharyngeal arch and outflow tract in RA treated groups. Real-time quantitative PCR analysis showed that Tbx1 expression rose to a peak level at 36 hpf in wild type group. Repression of Tbx1 expression was most evident at 36 hpf, 24 hours after RA treatment. 10(-7 )mol/L RA caused a more severe effect on the Tbx1 expression level than 5 x 10(-8)mol/L RA. The results suggested that RA could produce an altered Tbx1 expression pattern in zebrafish. In addition, RA could repress Tbx1 expression in a dose-dependent manner.
- Subjects :
- Animals
Branchial Region drug effects
Branchial Region embryology
Down-Regulation drug effects
Down-Regulation genetics
Embryo, Nonmammalian drug effects
Embryo, Nonmammalian physiology
In Situ Hybridization
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
T-Box Domain Proteins antagonists & inhibitors
T-Box Domain Proteins genetics
Tretinoin pharmacology
Zebrafish embryology
DNA-Binding Proteins antagonists & inhibitors
DNA-Binding Proteins genetics
DiGeorge Syndrome genetics
Gene Expression Regulation, Developmental drug effects
Tretinoin physiology
Zebrafish Proteins antagonists & inhibitors
Zebrafish Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0214-6282
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The International journal of developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 16323078
- Full Text :
- https://doi.org/10.1387/ijdb.052036lz