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Cyclin and caveolin expression in an acute model of murine Chagasic myocarditis.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2006 Jan; Vol. 5 (1), pp. 107-12. Date of Electronic Publication: 2006 Jan 28. - Publication Year :
- 2006
-
Abstract
- Chagas' disease caused by the parasite, Trypanosoma cruzi, is accompanied by an acute myocarditis which can be fatal. Mice (A/J strain) infected with T. cruzi (Tulahuen strain) develop an acute myocarditis associated with high parasitemia and uniform mortality. Examination of the myocardium demonstrated myonecrosis, vasculitis and parasite pseudocysts. Immunoblot analysis and quantitative real time PCR of heart lysates demonstrated an increased expression of cell cycle regulatory proteins such as cyclins B1, D1, A1 and E1 and an increased expression of cdk2 when compared with uninfected controls. Extracellular signal-regulated kinase (ERK) was activated. Proliferating cell nuclear antigen (PCNA), endothelin-1, endothelin receptor type A (ET(A)) and endothelin receptor type B (ET(B)) expression were increased. Caveolin-1 is important in the regulation of ERK and cyclin D1. The expression of caveolin-1 as well as caveolin-2 and caveolin-3 was reduced. These data suggest that acute fatal T. cruzi myocarditis is accompanied by changes in cell cycle proteins such as the cyclins and caveolin and that the upregulation of the endothelin pathway may be important in the myocardial abnormalities and mortality observed in this mouse model.
- Subjects :
- Acute Disease
Animals
Chagas Disease metabolism
Cyclins genetics
Disease Models, Animal
Endothelin-1 metabolism
Gene Expression Regulation
Male
Mice
Myocarditis genetics
Myocarditis parasitology
RNA, Messenger genetics
Trypanosoma cruzi physiology
Caveolin 1 metabolism
Chagas Disease complications
Cyclins metabolism
Myocarditis metabolism
Myocarditis pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 16319533
- Full Text :
- https://doi.org/10.4161/cc.5.1.2284