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Serotonin induces pulmonary artery smooth muscle cell migration.
- Source :
-
Biochemical pharmacology [Biochem Pharmacol] 2006 Jan 12; Vol. 71 (3), pp. 386-97. - Publication Year :
- 2006
-
Abstract
- The chronic phase of pulmonary arterial hypertension (PAH) is associated with vascular remodeling, especially thickening of the smooth muscle layer of large pulmonary arteries and muscularization of small pulmonary vessels, which normally have no associated smooth muscle. Serotonin (5-hydroxytryptamine, 5-HT) has been shown to induce proliferation and hypertrophy of pulmonary artery smooth muscle cells (PASMC), and may be important for in vivo pulmonary vascular remodeling. Here, we show that 5-HT stimulates migration of pulmonary artery PASMC. Treatment with 5-HT for 16h increased migration of PASMC up to four-fold as monitored in a modified Boyden chamber assay. Increased migratory responses were associated with cellular morphological changes and reorganization of the actin cytoskeleton. 5-HT-induced alterations in morphology were previously shown in our laboratory to require cAMP [Lee SL, Fanburg BL. Serotonin produces a configurational change of cultured smooth muscle cells that is associated with elevation of intracellular cAMP. J Cell Phys 1992;150(2):396-405], and the 5-HT4 receptor was pharmacologically determined to be the primary activator of cAMP in bovine PASMC [Becker BN, Gettys TW, Middleton JP, Olsen CL, Albers FJ, Lee SL, et al. 8-Hydroxy-2-(di-n-propylamino)tetralin-responsive 5-hydroxytryptamine4-like receptor expressed in bovine pulmonary artery smooth muscle cells. Mol Pharmacol 1992;42(5):817-25]. We examined the role of the 5-HT4 receptor and cAMP in 5-HT-induced bovine PASMC migration. PASMC express 5-HT4 receptor mRNA, and a 5-HT4 receptor antagonist and a cAMP antagonist completely blocked 5-HT-induced cellular migration. Consistent with our previous report that a cAMP-dependent Cl(-) channel is required for 5-HT-induced morphological changes in PASMC, phenylanthranilic acid, a Cl(-) channel blocker, inhibited actin cytoskeletal reorganization and migration produced by 5-HT. We conclude that 5-HT stimulates PASMC migration and associated cytoskeletal reorganization through the 5-HT4 receptor and cAMP activation of a chloride channel.
- Subjects :
- Animals
Blotting, Western
Cattle
Cells, Cultured
Cyclic AMP antagonists & inhibitors
Cyclic AMP biosynthesis
Muscle, Smooth, Vascular drug effects
Muscle, Smooth, Vascular metabolism
Pulmonary Artery drug effects
Pulmonary Artery metabolism
Receptors, Serotonin, 5-HT4 biosynthesis
Serotonin 5-HT4 Receptor Antagonists
Cell Movement drug effects
Muscle, Smooth, Vascular cytology
Pulmonary Artery cytology
Serotonin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-2952
- Volume :
- 71
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 16316635
- Full Text :
- https://doi.org/10.1016/j.bcp.2005.10.035