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Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor.
- Source :
-
Molecular microbiology [Mol Microbiol] 2005 Dec; Vol. 58 (5), pp. 1276-87. - Publication Year :
- 2005
-
Abstract
- A complex programme of regulation governs gene expression during development of the morphologically and biochemically complex eubacterial genus Streptomyces. Earlier work has suggested a model in which 'higher level' pleiotropic regulators activate 'pathway-specific' regulators located within chromosomal gene clusters encoding biosynthesis of individual antibiotics. We used mutational analysis and adventitious overexpression of key Streptomyces coelicolor regulators to investigate functional interactions among them. We report here that cluster-situated regulators (CSRs) thought to be pathway-specific can also control other antibiotic biosynthetic gene clusters, and thus have pleiotropic actions. Surprisingly, we also find that CSRs exhibit growth-phase-dependent control over afsR2/afsS, a 'higher level' pleiotropic regulatory locus not located within any of the chromosomal gene clusters it targets, and further demonstrate that cross-regulation by CSRs is modulated globally and differentially during the S. coelicolor growth cycle by the RNaseIII homologue AbsB. Our results, which reveal a network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor, suggest that revision of the currently prevalent view of higher-level versus pathway-specific regulation of secondary metabolism in Streptomyces species is warranted.
- Subjects :
- Bacterial Proteins genetics
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Mutation
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Streptomyces coelicolor genetics
Streptomyces coelicolor growth & development
Transcription Factors genetics
Transcription Factors metabolism
Anti-Bacterial Agents biosynthesis
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Genes, Regulator
Multigene Family
Streptomyces coelicolor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0950-382X
- Volume :
- 58
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16313616
- Full Text :
- https://doi.org/10.1111/j.1365-2958.2005.04879.x