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Characterization of vibrio parahaemolyticus isolated from coastal seawater in peninsular Malaysia.
Characterization of vibrio parahaemolyticus isolated from coastal seawater in peninsular Malaysia.
- Source :
-
The Southeast Asian journal of tropical medicine and public health [Southeast Asian J Trop Med Public Health] 2005 Jul; Vol. 36 (4), pp. 940-5. - Publication Year :
- 2005
-
Abstract
- Twenty-one Vibrio parahaemolyticus isolates representing 21 samples of coastal seawater from three beaches in peninsular Malaysia were found to be sensitive to streptomycin, norfloxacin and chloramphenicol. Resistance was observed to penicillin (100%), ampicillin (95.2%), carbenicilin (95.2%), erythromycin (95.2%), bacitracin (71.4%), cephalothin (28.6%), moxalactam (28.6%), kanamycin (19.1%), tetracycline (14.3%), nalidixic acid (9.5%) and gentamicin (9.5%). Plasmids of 2.6 to 35.8 mDa were detected among plasmid-containing isolates. All isolates carried the Vp-toxR gene specific to V. parahaemolyticus and were negative for the tdh gene, but only one isolate was positive for the trh gene. DNA fingerprinting of the isolates using ERIC-PCR and PFGE showed that the isolates belong to two major clonal groups, with several isolates from different locations in the same group, indicating the presence of similar strains in the different locations.
- Subjects :
- Animals
Base Sequence
DNA Fingerprinting
DNA Primers
Drug Resistance, Microbial
Electrophoresis, Gel, Pulsed-Field
Humans
Malaysia
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
Vibrio Infections microbiology
Vibrio parahaemolyticus isolation & purification
Vibrio parahaemolyticus classification
Vibrio parahaemolyticus pathogenicity
Water Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0125-1562
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Southeast Asian journal of tropical medicine and public health
- Publication Type :
- Academic Journal
- Accession number :
- 16295549