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Regulation of vascular smooth muscle cell proliferation, migration and death by heparan sulfate 6-O-endosulfatase1.
- Source :
-
FEBS letters [FEBS Lett] 2005 Nov 21; Vol. 579 (28), pp. 6493-8. Date of Electronic Publication: 2005 Nov 02. - Publication Year :
- 2005
-
Abstract
- Migration, proliferation and death of vascular smooth muscle cells (VSMC) are important events in vascular pathology regulated by heparan sulfate proteoglycans and hence potentially by cell surface HS 6-O-endosulfatase1 (sulf1). Sulf1 mRNA expression was increased in cultured VSMC compared to rat aorta. Furthermore, adenovirus mediated overexpression of quail sulf1 decreased adhesion, and increased proliferation and apoptosis of VSMC. Overexpression of a dominant negative variant also decreased adhesion of VSMC and increased proliferation, apoptosis, migration and chemotaxis of VSMC. Our results imply that only normal levels of 6-O-sulfation maintained by sulf1 are optimal for several functions of VSMC.
- Subjects :
- Adenoviridae genetics
Animals
Aorta enzymology
Apoptosis
Cell Movement
Cell Proliferation
Focal Adhesion Protein-Tyrosine Kinases metabolism
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 metabolism
Muscle, Smooth, Vascular cytology
Mutation genetics
Myocytes, Smooth Muscle drug effects
Myocytes, Smooth Muscle physiology
Phosphorylation
RNA, Messenger analysis
RNA, Messenger metabolism
Rats
Sulfatases genetics
Sulfatases metabolism
Sulfotransferases genetics
Muscle, Smooth, Vascular enzymology
Myocytes, Smooth Muscle enzymology
Sulfotransferases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 579
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 16289059
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.10.026