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Isolation and proteomic analysis [corrected] of cell wall-deficient Haematococcus pluvialis mutants.
- Source :
-
Proteomics [Proteomics] 2005 Dec; Vol. 5 (18), pp. 4839-51. - Publication Year :
- 2005
-
Abstract
- The green alga Haematococcus pluvialis has a plant-like cell wall consisting of glycoproteins and cellulose that is modified during the cell cycle and under various conditions. These features allow Haematococcus to be used as a model organism for studying cell wall biology. Development of the Haematococcus model is hampered by the absence of mutants that could provide insight into the biosynthesis and assembly of wall components. Haematococcus mutants (WM#537 and WM#2978) (WM--wall mutant) with defective cell walls were obtained by chemical mutagenesis. WM#537 features a secondary wall of considerably reduced thickness, whereas WM#2978 possesses a somewhat reduced secondary wall with little intervening space between the wall and plasmalemma. 2-DE revealed that a majority of the cell wall proteins were present in the wild-type and mutant cell walls throughout the cell cycle. PMF identified 55 wall protein orthologs from these strains, including a subset of induced proteins known to be involved in wall construction, remodeling, and defense. Down-regulation of certain wall proteins in the two mutants was associated with the wall defects, whereas overexpression of other proteins may have compensated for the defective walls in the two mutants.
- Subjects :
- Algal Proteins biosynthesis
Cell Wall ultrastructure
Chlorophyta drug effects
Chlorophyta genetics
Chlorophyta metabolism
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Gene Expression Profiling
Methylnitronitrosoguanidine pharmacology
Mutation
Up-Regulation
Algal Proteins chemistry
Cell Wall metabolism
Chlorophyta chemistry
Chlorophyta ultrastructure
Proteome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9853
- Volume :
- 5
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 16281177
- Full Text :
- https://doi.org/10.1002/pmic.200400092