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Effects of probiotic bacteria (VSL#3) on the polyamine biosynthesis and cell proliferation of normal colonic mucosa of rats.
- Source :
-
In vivo (Athens, Greece) [In Vivo] 2005 Nov-Dec; Vol. 19 (6), pp. 989-95. - Publication Year :
- 2005
-
Abstract
- Background: Probiotics seem to possess tumour inhibitory properties, but few studies have investigated their actions on the cell proliferation of normal colonic mucosa. The effects of a probiotic mixture (VSL#3) on polyamine biosynthesis, Ki-67 levels and apoptosis in the normal colon of rats were studied.<br />Materials and Methods: For a 4-week period, 20 rats were fed a VSL#3 solution and 20 rats a saline solution. Samples from the colonic mucosa were collected at the end of treatment. Polyamines were detected by HPLC, ornithine decarboxylase activity by a radiometric technique, and apoptosis and Ki-67 by histochemical and immunohistochemical methods.<br />Results: VSL#3 caused a significant decrease in colonic polyamine levels, ornithine decarboxylase activity and Ki-67 compared to controls. A significant increase in the apoptotic index was also observed.<br />Conclusion: Probiotics could also reduce proliferation rates in a condition not affected by hyperproliferative or neoplastic growth, when the normal control mechanisms are still completely effective.
- Subjects :
- Animals
Apoptosis drug effects
Chromatography, High Pressure Liquid
Colon anatomy & histology
Colon drug effects
Histocytochemistry
Immunohistochemistry
Intestinal Mucosa enzymology
Intestinal Mucosa metabolism
Ki-67 Antigen analysis
Ki-67 Antigen metabolism
Male
Ornithine Decarboxylase analysis
Ornithine Decarboxylase metabolism
Polyamines metabolism
Random Allocation
Rats
Rats, Inbred F344
Spermidine analysis
Spermine analysis
Time Factors
Bifidobacterium physiology
Cell Proliferation drug effects
Intestinal Mucosa microbiology
Lactobacillus physiology
Polyamines analysis
Probiotics pharmacology
Streptococcus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0258-851X
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- In vivo (Athens, Greece)
- Publication Type :
- Academic Journal
- Accession number :
- 16277012