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Cloning and alternative splicing analysis of Bombyx mori transformer-2 gene using silkworm EST database.
- Source :
-
Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2005 Nov; Vol. 37 (11), pp. 728-36. - Publication Year :
- 2005
-
Abstract
- We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence, it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins.
- Subjects :
- Alternative Splicing genetics
Amino Acid Sequence
Animals
Cloning, Molecular
Conserved Sequence
Databases, Genetic
Drosophila Proteins chemistry
Expressed Sequence Tags
Female
Male
Molecular Sequence Data
Organ Specificity
Ribonucleoproteins chemistry
Sequence Alignment methods
Sequence Analysis, Protein methods
Sequence Homology, Amino Acid
Tissue Distribution
Bombyx genetics
Bombyx metabolism
Chromosome Mapping methods
Drosophila Proteins genetics
Drosophila Proteins metabolism
Ribonucleoproteins genetics
Ribonucleoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1672-9145
- Volume :
- 37
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Acta biochimica et biophysica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 16270151
- Full Text :
- https://doi.org/10.1111/j.1745-7270.2005.00106.x