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Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system.

Authors :
Macfarlane S
Woodmansey EJ
Macfarlane GT
Source :
Applied and environmental microbiology [Appl Environ Microbiol] 2005 Nov; Vol. 71 (11), pp. 7483-92.
Publication Year :
2005

Abstract

The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus breakdown. The aims of this study were to determine how intestinal bacteria colonize mucus and to study physiologic and enzymatic factors involved in the destruction of this glycoprotein. Colonization of mucin gels by fecal bacteria was studied in vitro, using a two-stage continuous culture system, simulating conditions of nutrient availability and limitation characteristic of the proximal (vessel 1) and distal (vessel 2) colon. The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes. Gel samples were also taken for analysis of mucin-degrading enzymes and measurements of residual mucin sugars. Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia. Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.

Details

Language :
English
ISSN :
0099-2240
Volume :
71
Issue :
11
Database :
MEDLINE
Journal :
Applied and environmental microbiology
Publication Type :
Academic Journal
Accession number :
16269790
Full Text :
https://doi.org/10.1128/AEM.71.11.7483-7492.2005