Back to Search
Start Over
Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2005 Nov; Vol. 71 (11), pp. 7483-92. - Publication Year :
- 2005
-
Abstract
- The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus breakdown. The aims of this study were to determine how intestinal bacteria colonize mucus and to study physiologic and enzymatic factors involved in the destruction of this glycoprotein. Colonization of mucin gels by fecal bacteria was studied in vitro, using a two-stage continuous culture system, simulating conditions of nutrient availability and limitation characteristic of the proximal (vessel 1) and distal (vessel 2) colon. The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes. Gel samples were also taken for analysis of mucin-degrading enzymes and measurements of residual mucin sugars. Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia. Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.
- Subjects :
- Bacteria, Anaerobic classification
Bacteria, Anaerobic genetics
Bacteriological Techniques instrumentation
Bacteriological Techniques methods
Culture Media
Ecosystem
Fluorescent Dyes
Humans
Microscopy, Confocal
Microscopy, Electron, Scanning
Oligonucleotide Probes
Plankton growth & development
RNA, Ribosomal, 16S genetics
Bacteria, Anaerobic growth & development
Biofilms growth & development
Feces microbiology
Intestine, Large microbiology
Mucins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 71
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16269790
- Full Text :
- https://doi.org/10.1128/AEM.71.11.7483-7492.2005