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Mass spectrometric identification and quantification of glycosyl flavonoids, including dihydrochalcones with neutral loss scan mode.
- Source :
-
Analytical biochemistry [Anal Biochem] 2005 Dec 15; Vol. 347 (2), pp. 182-92. Date of Electronic Publication: 2005 Oct 07. - Publication Year :
- 2005
-
Abstract
- We developed a strategy for determination and quantification of glycosyl flavonoids using liquid chromatography-triple quadrupole mass spectrometry with neutral loss scan at 15 and 30eV collision energy in the positive ion mode. The fragmentation patterns of glycosyl flavonoids at 15 and 30eV showed that fragmentation of sugar moiety depended on the type of glycosidic bond to aglycone, the site of C-glycosylation, and the type of aglycone. C-Glycosyl dihydrochalcones especially stood out because they produced [M+H-162](+) even at 15eV such as O-glycoside in spite of C-glycoside. C-Glycosides were classified according to (i) the intensity ratio A of [M+H-150](+) to [M+H-120](+) at 30eV and (ii) the intensity ratio B of [M+H-120](+) at 15eV to one at 30eV. The 8-C-glycosides were A<1 and B<1, the 6-C-glycosides were A>1 and B<1, and the C-glycosyl dihydrochalcones were A>1 and B>>1. Therefore, the intensity ratios of the neutral loss scan of 120 and 150Da at 30eV and those of 120, 162, and 308Da at 15eV allowed sequential distinction among these three types of C-glycosides as well as between O- and C-glycosides. Our method was applied for analysis of Rooibos tea, and the identified glycosides could be quantified specifically by the selected reaction monitoring method.
- Subjects :
- Aspalathus chemistry
Beverages analysis
Chalcone analysis
Chalcone chemistry
Chalcones analysis
Chalcones chemistry
Chemistry Techniques, Analytical
Chromatography, High Pressure Liquid
Flavonoids chemistry
Gas Chromatography-Mass Spectrometry
Glycosylation
Mass Spectrometry statistics & numerical data
Molecular Structure
Chalcone analogs & derivatives
Flavonoids analysis
Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 347
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16269127
- Full Text :
- https://doi.org/10.1016/j.ab.2005.09.020