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Co-expression of the 5-HT(3B) subunit with the 5-HT(3A) receptor reduces alcohol sensitivity.
- Source :
-
Brain research. Molecular brain research [Brain Res Mol Brain Res] 2005 Dec 14; Vol. 142 (2), pp. 146-50. Date of Electronic Publication: 2005 Oct 28. - Publication Year :
- 2005
-
Abstract
- Allosteric modulation of mouse 5-Hydroxytryptamine(3A) (5-HT(3A)) and 5-HT(3A/B) receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT(3A) receptor function, but had no effect on mouse 5-HT(3A/B) receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT(3A/B) receptor function was much less than that observed in the mouse 5-HT(3A) receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20-80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT(3) receptors.
- Subjects :
- Animals
Cell Line
Dose-Response Relationship, Drug
Electric Stimulation methods
Ethylene Chlorohydrin analogs & derivatives
Ethylene Chlorohydrin pharmacology
Humans
Membrane Potentials drug effects
Membrane Potentials physiology
Membrane Potentials radiation effects
Mice
Patch-Clamp Techniques methods
Protein Subunits genetics
Protein Subunits metabolism
Receptors, Serotonin, 5-HT3 genetics
Serotonin pharmacology
Transfection methods
Central Nervous System Depressants pharmacology
Ethanol pharmacology
Gene Expression drug effects
Receptors, Serotonin, 5-HT3 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0169-328X
- Volume :
- 142
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brain research. Molecular brain research
- Publication Type :
- Academic Journal
- Accession number :
- 16257471
- Full Text :
- https://doi.org/10.1016/j.molbrainres.2005.09.011