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[The effect of hIL-4 on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2005 Nov; Vol. 21 (6), pp. 775-7. - Publication Year :
- 2005
-
Abstract
- Aim: To investigate the effect of human interleukin-4 (hIL-4) on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells(BAEC) activated with TNF-alpha.<br />Methods: BAEC were cultured in different concentrations of hIL-4 for 2 h followed by treatment with 4 microg/L TNF-alpha for 6 h or 18 h. The expression of E-selectin and ICAM-1 on BAEC was detected by cell-ELISA. The effect of hIL-4 on viability of BAEC was detected by MTT colorimetry.<br />Results: Pretreatment of BAEC with hIL-4 inhibited the expression of E-selectin and ICAM-1 on BAEC activated by TNF-alpha in a dose-dependent manner (P<0.01). Both TNF-alpha and hIL-4 had no effect on the viability of BAEC.<br />Conclusion: hIL-4 can suppress the expression of E-selectin and ICAM-1 on BAEC activated by TNF-alpha, which may contribute to the xenotransplant immune tolerance associated with hIL-4.
- Subjects :
- Animals
Cattle
Cell Survival drug effects
Cells, Cultured
Endothelial Cells drug effects
Enzyme-Linked Immunosorbent Assay
Humans
Immunohistochemistry
Tumor Necrosis Factor-alpha pharmacology
Adjuvants, Immunologic pharmacology
Aorta cytology
E-Selectin metabolism
Endothelial Cells metabolism
Gene Expression Regulation drug effects
Intercellular Adhesion Molecule-1 metabolism
Interleukin-4 pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 21
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16256043