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[Detection of antigen-antibody interaction of human adenovirus by the method of surface plasmon resonance].

Authors :
Nosach LM
Boltovets' PM
Povnytsia OIu
Zhovnovata VL
Zakharenko OM
Snopok BA
Shyrshov IuM
Diachenko NS
Source :
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) [Mikrobiol Z] 2005 Jul-Aug; Vol. 67 (4), pp. 58-64.
Publication Year :
2005

Abstract

A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of antihexon antibodies was demonstrated by indirect method of fluorescent antibodies (MFA) and cellular variant of the immunoassay (cELISA).

Details

Language :
Ukrainian
ISSN :
1028-0987
Volume :
67
Issue :
4
Database :
MEDLINE
Journal :
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993)
Publication Type :
Academic Journal
Accession number :
16250237