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[Study on mechanism of the anti-tumor activity of Acanthopanax gracilistylus].
- Source :
-
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine [Zhongguo Zhong Xi Yi Jie He Za Zhi] 2005 Sep; Vol. 25 (9), pp. 825-8. - Publication Year :
- 2005
-
Abstract
- Objective: To study the mechanism of anti-tumor activity of Acanthopanax gracilistylus extract (Age).<br />Methods: The tumor cells proliferation was detected by using (3H)-TdR incorporation method, and the effects of Age on cell cycle of tumor cells, retinoblastoma (Rb) protein and cyclin-dependent kinases (Cdk) were analyzed by flow cytometry and Western blotting assay, respectively.<br />Results: It was indicated by cytoactivity test in vitro that Age only had effect in inhibiting the proliferation of tumor cells, it couldn't lead to death of cells. Under action of Age, the proliferation of tumor cells was halted at G0/G1 stage of cell cycle, and showed no direct cytotoxic effect by Age. Age could induce lowering of the expression of Rb, Cdk2 and Cdk4, cause halt of tumor cell proliferation.<br />Conclusion: The tumor inhibitory effect of Age is realized by way of regulating the activity of cell cycle controlling enzymes to suspend the proliferation of tumor cells.
- Subjects :
- Adenocarcinoma pathology
Cell Proliferation drug effects
Cyclin-Dependent Kinase 2 biosynthesis
Cyclin-Dependent Kinase 4 biosynthesis
Humans
Leukemia, T-Cell pathology
Mouth Neoplasms pathology
Retinoblastoma Protein biosynthesis
Stomach Neoplasms pathology
Tumor Cells, Cultured
Antineoplastic Agents, Phytogenic pharmacology
Drugs, Chinese Herbal pharmacology
Eleutherococcus chemistry
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1003-5370
- Volume :
- 25
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
- Publication Type :
- Academic Journal
- Accession number :
- 16248248